RNase Free Certification

The RNase Detection Assay will test for the presence of common environmental RNases (human, bacterial, and viral) and qualify products tested to be safe for use in RNA related work. Passing lots are accompanied with a certificate of conformity and considered to be RNase Free below a 10⁻¹¹ Kunitz Units sensitivity.

The test is commonly performed in conjunction with the DNase Detection Assay as an indicator of the cleanliness of the manufacturing and efficacy of sterilization programs.

The ubiquity of RNase is a huge concern for researchers in RNA work. Its pervasiveness as an environmental contaminant can be attributed to humans. It is exuded from the sebaceous oils of the human epidermis, and humans are the primary source for ribonucleases. Environmental contamination is also of great concern, as microorganisms are a significant contributor to RNase as well. RNase is a robust enzyme capable of withstanding prolonged sterilization and resistant to chemical inactivation. Therefore it is of the utmost importance that precautions be taken to ensure that glassware, solutions, reagents, workspace, tools, and gloves are free from ribonuclease contamination when working with RNA.